8BZ9
single soak stabilizer for ERa - 14-3-3 interaction (AZ354)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PETRA III, DESY BEAMLINE P11 |
| Synchrotron site | PETRA III, DESY |
| Beamline | P11 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-05 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 1.033200 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 81.764, 111.602, 62.364 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.310 - 1.300 |
| R-factor | 0.1846 |
| Rwork | 0.184 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.951 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.19.2-4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 65.960 | 1.320 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Number of reflections | 369808 | 2155 |
| <I/σ(I)> | 13.6 | |
| Completeness [%] | 95.8 | |
| Redundancy | 5.5 | |
| CC(1/2) | 0.998 | 0.367 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | 0.095 M HEPES (pH 7.1 ), PEG400 (29% (v/v)), 0.19 M CaCl2 and 5% (v/v) Glycerol |
