8BZ5
Crystal structure of the L. monocytogenes RmlT in complex with HEPES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 2 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-09 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.980112 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 82.081, 178.367, 92.154 |
| Unit cell angles | 90.00, 93.52, 90.00 |
Refinement procedure
| Resolution | 48.120 - 2.300 |
| R-factor | 0.1944 |
| Rwork | 0.193 |
| R-free | 0.22020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8bz4 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.586 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.120 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.112 | 1.295 |
| Rmeas | 0.121 | 1.396 |
| Rpim | 0.046 | 0.519 |
| Number of reflections | 116033 | 5688 |
| <I/σ(I)> | 10.7 | 1.4 |
| Completeness [%] | 99.2 | 98.4 |
| Redundancy | 7 | 7.2 |
| CC(1/2) | 0.998 | 0.596 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M HEPES-NaOH pH 7.5 20% (w/v) PEG 8,000 |
