8BXX

Crystal structure of formate dehydrogenase FDH2 enzyme from Granulicella mallensis MP5ACTX8 in complex with NAD and azide.

This is a non-PDB format compatible entry.

Replaces:  6T8J

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-06-06
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.99987
Spacegroup name	P 1 21 1
Unit cell lengths	72.066, 128.810, 85.664
Unit cell angles	90.00, 100.40, 90.00

Refinement procedure

Resolution	62.100 - 1.970
Rwork	0.188
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	xia2
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	62.100	2.040
High resolution limit [Å]	1.970	1.970
Number of reflections	107087	10633
<I/σ(I)>	8.96	2.31
Completeness [%]	97.8
Redundancy	3.5
CC(1/2)	0.913	0.551

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	5.5	293	0,1 M Ammonium Sulfate 0.1 M Bis-Tris pH 5.5 25% PEG 3350 5 mM NaN3, 10 mM NAD