8BXA

Crystal structure of ribosome binding factor A (RbfA) from S. aureus

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	BESSY BEAMLINE 14.1
Synchrotron site	BESSY
Beamline	14.1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-10-27
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	0.991872
Spacegroup name	P 41
Unit cell lengths	65.315, 65.315, 27.287
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	46.180 - 2.220
R-factor	0.201
Rwork	0.199
R-free	0.24740
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.009
RMSD bond angle	1.006
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	46.180	2.300
High resolution limit [Å]	2.220	2.220
Rmerge	0.059	0.783
Rmeas	0.063	0.847
Rpim	0.023	0.320
Number of reflections	5910	592
<I/σ(I)>	18.6	2.44
Completeness [%]	99.9	100
Redundancy	7.2	6.9
CC(1/2)	0.999	0.893

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	0.1 M MES pH 6.5, 80 mM Manganese(II) chloride, 15% PEG 20000