8BVP
Crystal structure of an N-terminal fragment of the effector protein Lpg2504 (SidI) from Legionella pneumophila
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-17 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.978565 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 108.700, 61.030, 113.490 |
| Unit cell angles | 90.00, 107.29, 90.00 |
Refinement procedure
| Resolution | 36.120 - 2.100 |
| R-factor | 0.2091 |
| Rwork | 0.207 |
| R-free | 0.25410 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.795 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.120 | 2.200 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.037 | 0.897 |
| Number of reflections | 41606 | 5384 |
| <I/σ(I)> | 23.42 | |
| Completeness [%] | 99.7 | 100 |
| Redundancy | 6.9 | 2.04 |
| CC(1/2) | 1.000 | 0.846 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 291 | 100 mM Tris-HCl ph 8.5, 14 % ethanol |
