8BVI

Crystal structure of the METTL9-like histidine methyltransferase from Ostreococcus tauri

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-10-05
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.918402
Spacegroup name	P 21 3
Unit cell lengths	106.898, 106.898, 106.898
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.810 - 3.100
R-factor	0.2258
Rwork	0.223
R-free	0.28010
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.001
RMSD bond angle	0.332
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.590	3.310
High resolution limit [Å]	3.100	3.100
Rmerge	0.117	1.900
Rmeas	0.124	2.010
Rpim	0.041	0.648
Number of reflections	7642	1372
<I/σ(I)>	10.5	1.3
Completeness [%]	100.0	100
Redundancy	9.4	9.4
CC(1/2)	0.997	0.553

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	277	0.1 M Hepes pH 7.5, 0.2 M L-proline, 10% w/v polyethylene glycol 3,350