8BVB
Crystal structure of the apo form of SmbA loop deletion mutant.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-05-31 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97319 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 61.375, 208.105, 64.234 |
| Unit cell angles | 90.00, 117.58, 90.00 |
Refinement procedure
| Resolution | 56.940 - 1.800 |
| R-factor | 0.1706 |
| Rwork | 0.169 |
| R-free | 0.20310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6gs8 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.051 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 56.940 | 1.864 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.075 | 0.555 |
| Rmeas | 0.090 | 0.664 |
| Rpim | 0.049 | 0.361 |
| Number of reflections | 128620 | 12871 |
| <I/σ(I)> | 12.06 | 2.86 |
| Completeness [%] | 97.8 | 97.84 |
| Redundancy | 3.3 | 3.3 |
| CC(1/2) | 0.996 | 0.763 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 303 | 200 mM NaCl and 10 % v/w PEG 6000 |
