8BV3
Bacillus subtilis DnaA domain III structure
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | DIAMOND BEAMLINE I24 |
Synchrotron site | Diamond |
Beamline | I24 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-25 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.99987 |
Spacegroup name | P 32 |
Unit cell lengths | 81.578, 81.578, 223.196 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 74.399 - 2.380 |
R-factor | 0.1931 |
Rwork | 0.191 |
R-free | 0.23870 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1l8q |
Data reduction software | autoPROC (1.0.5 (20211020)) |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 74.399 | 2.520 |
High resolution limit [Å] | 2.380 | 2.380 |
Rmerge | 0.179 | 1.461 |
Rmeas | 0.189 | 1.583 |
Rpim | 0.060 | 0.599 |
Number of reflections | 56743 | 2842 |
<I/σ(I)> | 10.1 | 1.5 |
Completeness [%] | 85.3 | 27.2 |
Redundancy | 9.7 | 6.7 |
CC(1/2) | 0.996 | 0.461 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | EVAPORATION | 293.15 | Morpheus H3: 0.02 M DL-Glutamic acid monohydrate, 0.02 M DL-Alanine, 0.0 2M Glycine, 0.2M DL-Lysine monohydrochloride, 0.02M DL-Serine, 0.1 M Imidazole/ MES monohydrate pH 6.5, 20% v/v Glycerol, 20% w/v PEG 4000 |