8BUS
Structure of DDB1 bound to DS59-engaged CDK12-cyclin K
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-06-27 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 249.522, 249.522, 218.546 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 60.400 - 3.260 |
| R-factor | 0.2091 |
| Rwork | 0.208 |
| R-free | 0.22820 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.722 |
| Data reduction software | XDS |
| Data scaling software | STARANISO |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 216.091 | 3.430 |
| High resolution limit [Å] | 3.253 | 3.253 |
| Number of reflections | 102328 | 5114 |
| <I/σ(I)> | 12.4 | |
| Completeness [%] | 94.2 | |
| Redundancy | 20.8 | |
| CC(1/2) | 0.998 | 0.379 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 1.40 M ammonium sulphate, 0.07 M HEPES, pH 7.0 |
