8BUP

Structure of DDB1 bound to DS30-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	248.058, 248.058, 221.169
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	57.530 - 3.410
R-factor	0.2014
Rwork	0.200
R-free	0.22280
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.877
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	214.825	3.586
High resolution limit [Å]	3.408	3.408
Number of reflections	92873	4648
<I/σ(I)>	13.6
Completeness [%]	96.0
Redundancy	21
CC(1/2)	0.999	0.372

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.44 M ammonium sulphate, 2.143 %v/v PEG 200, 0.07 M HEPES pH 7.5