8BUE

Structure of DDB1 bound to Z11-engaged CDK12-cyclin K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-09-21
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 31 2 1
Unit cell lengths	249.329, 249.329, 218.681
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	60.410 - 3.250
R-factor	0.1857
Rwork	0.184
R-free	0.21310
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.006
RMSD bond angle	0.883
Data reduction software	XDS
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	215.925	3.450
High resolution limit [Å]	3.246	3.246
Number of reflections	98057	4908
<I/σ(I)>	14.4
Completeness [%]	95.9
Redundancy	21
CC(1/2)	0.999	0.414

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.45 M ammonium sulphate, 0.07 M HEPES, pH 7.1