8BS9

Structure of USP36 in complex with Ubiquitin-PA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-02-14
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	0.999860
Spacegroup name	P 1 21 1
Unit cell lengths	75.743, 65.307, 103.411
Unit cell angles	90.00, 93.27, 90.00

Refinement procedure

Resolution	45.240 - 1.900
R-factor	0.173
Rwork	0.171
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.010
RMSD bond angle	1.007
Data reduction software	XDS (VERSION Jan 10, 2022 BUILT=20220110)
Data scaling software	Aimless (0.7.7)
Phasing software	PHASER (2.8.3)
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	45.240	1.940
High resolution limit [Å]	1.900	1.900
Rmerge	0.066	0.999
Number of reflections	152836	15177
<I/σ(I)>	11.8	1.4
Completeness [%]	99.9	100
Redundancy	4	4.2
CC(1/2)	0.999	0.604

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8	291	0.3 M potassium formate, 14% (w/v) PEG 3350