8BS8

Bovine naive ultralong antibody AbD08 collected at 100K

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I04
Synchrotron site	DIAMOND
Beamline	I04
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-09
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9795
Spacegroup name	P 21 21 21
Unit cell lengths	63.500, 71.110, 98.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.640 - 1.590
R-factor	0.17683
Rwork	0.174
R-free	0.21889
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.478
Data reduction software	xia2 (0.6.277-g54103976-dials-2.0)
Data scaling software	pointless (1.11.21)
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	57.640	1.620
High resolution limit [Å]	1.590	1.590
Rmerge	0.058	1.307
Rmeas	0.063	1.416
Rpim	0.024	0.541
Total number of observations	408647	20025
Number of reflections	60610	2943
<I/σ(I)>	13.5	1.2
Completeness [%]	99.9
Redundancy	6.7	6.8
CC(1/2)	0.999	0.620

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	298	0.1M Tris (base) 0.1M Bicine 12% (v/v) PEG 500 MME 6% (v/v) PEG 20000 0.15M Sodium chloride 0.09M Sodium nitrate 0.09M Sodium phosphate dibasic 0.09M Ammonium sulphate