8BS5

Crystal structure of YTHDF3 YTH domain open conformation

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-11-15
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1
Spacegroup name	P 4 21 2
Unit cell lengths	80.860, 80.860, 122.340
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.770 - 2.490
R-factor	0.2765
Rwork	0.275
R-free	0.30560
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.007
RMSD bond angle	0.888
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.730	2.640
High resolution limit [Å]	2.490	2.490
Number of reflections	14732	2250
<I/σ(I)>	7.23
Completeness [%]	99.5
Redundancy	12.81
CC(1/2)	0.994	0.770

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	0.2M Potassium sodium tartrate tetrahydrate, 20% PEG 3350