8BRU
Escherichia coli methionyl-tRNA synthetase mutant L13M,I297C
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SOLEIL BEAMLINE PROXIMA 2 |
Synchrotron site | SOLEIL |
Beamline | PROXIMA 2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-08 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 78.310, 45.080, 86.170 |
Unit cell angles | 90.00, 107.54, 90.00 |
Refinement procedure
Resolution | 49.000 - 1.550 |
R-factor | 0.1461 |
Rwork | 0.144 |
R-free | 0.18060 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.007 |
RMSD bond angle | 0.839 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.000 | 1.640 |
High resolution limit [Å] | 1.540 | 1.540 |
Rmeas | 0.079 | |
Number of reflections | 83751 | 12534 |
<I/σ(I)> | 12.8 | 1.13 |
Completeness [%] | 98.6 | |
Redundancy | 6.6 | |
CC(1/2) | 0.999 | 0.636 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 277 | 1.08M ammonium citrate, 30 mM potassium phosphate |