8BR4
Structure of GAPDH from Mycobacterium tuberculosis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2016-09-14 |
| Detector | MAR scanner 345 mm plate |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 140.886, 139.166, 141.478 |
| Unit cell angles | 90.00, 108.52, 90.00 |
Refinement procedure
| Resolution | 47.150 - 3.290 |
| R-factor | 0.2571 |
| Rwork | 0.255 |
| R-free | 0.29550 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1gd1 |
| RMSD bond length | 0.001 |
| RMSD bond angle | 0.427 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.290 | 3.340 |
| High resolution limit [Å] | 3.280 | 3.280 |
| Rmerge | 0.136 | 0.517 |
| Rmeas | 0.179 | 0.681 |
| Rpim | 0.114 | 0.439 |
| Number of reflections | 78590 | 3434 |
| <I/σ(I)> | 10.3 | 2.5 |
| Completeness [%] | 98.5 | 76.5 |
| Redundancy | 4.5 | 4.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293.15 | 0.1 M Tris-HCl pH 8.0, 28% PEG 400 |
