8BQU
Molecular basis of ZP3/ZP1 heteropolymerization: crystal structure of a native vertebrate egg coat filament
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-29 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.97938 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 59.059, 59.059, 473.919 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 51.147 - 2.700 |
| R-factor | 0.25 |
| Rwork | 0.247 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Ensemble derived from PDB ID 7ZBM |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.604 |
| Data reduction software | XDS (Jan 10, 2022) |
| Data scaling software | Aimless (0.7.9) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | PHENIX (dev_4742) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 51.147 | 51.147 | 2.745 |
| High resolution limit [Å] | 2.419 | 9.658 | 2.419 |
| Rmerge | 0.199 | 0.047 | 0.615 |
| Rmeas | 0.225 | 0.054 | 0.734 |
| Rpim | 0.102 | 0.025 | 0.389 |
| Total number of observations | 63019 | 2710 | 2198 |
| Number of reflections | 14348 | 716 | 720 |
| <I/σ(I)> | 6.17 | 18.05 | 2.17 |
| Completeness [%] | 89.9 | 97 | 70 |
| Completeness (spherical) [%] | 37.3 | 97.0 | 6.1 |
| Completeness (ellipsoidal) [%] | 89.9 | 97.0 | 70.0 |
| Redundancy | 4.39 | 3.78 | 3.05 |
| CC(1/2) | 0.994 | 0.998 | 0.720 |
| Anomalous completeness (spherical) | 33.0 | 99.4 | 4.1 |
| Anomalous completeness | 88.6 | 99.4 | 61.9 |
| Anomalous redundancy | 2.6 | 2.8 | 1.9 |
| CC(ano) | 0.026 | 0.279 | 0.006 |
| |DANO|/σ(DANO) | 0.8 | 0.8 | 0.8 |
| Diffraction limits | Principal axes of ellipsoid fitted to diffraction cut-off surface |
| 3.960 Å | 1.000, 1.000, 1.000 |
| 3.960 Å | 0.000, 0.000, 0.000 |
| 2.257 Å | 0.000, 0.000, 0.000 |
| Criteria used in determination of diffraction limits | local <I/sigmaI> ≥ 1.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8 | 277.15 | 22% (w/v) PEG 3350, 0.2 M sodium/potassium phosphate, 0.1 M Tris-HCl pH 8.0 |
