8BQC
CjCel5B endo-glucanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-04-03 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9763 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 45.339, 53.481, 63.224 |
| Unit cell angles | 90.00, 109.75, 90.00 |
Refinement procedure
| Resolution | 42.050 - 1.570 |
| Rwork | 0.184 |
| R-free | 0.22670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1tvn |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.338 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 42.050 | 1.600 |
| High resolution limit [Å] | 1.570 | 1.570 |
| Rmerge | 0.147 | 2.693 |
| Rmeas | 0.160 | 2.910 |
| Rpim | 0.062 | 1.093 |
| Number of reflections | 35379 | 1983 |
| <I/σ(I)> | 7.7 | 0.7 |
| Completeness [%] | 88.5 | 100 |
| Redundancy | 6.5 | 6.9 |
| CC(1/2) | 0.998 | 0.320 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 277 | PS: 10 mg/mL in 5 mM Na-MOPS pH 7.5, 25 mM NaCl 1:1 with WS containing 0.2 M LiCl, 20% PEG3350 |
