8BPZ
Crystal structure of YopR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALBA BEAMLINE XALOC |
| Synchrotron site | ALBA |
| Beamline | XALOC |
| Temperature [K] | 80 |
| Detector technology | PIXEL |
| Collection date | 2021-05-05 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97934 |
| Spacegroup name | P 3 2 1 |
| Unit cell lengths | 109.081, 109.081, 53.908 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 94.470 - 2.200 |
| R-factor | 0.19967 |
| Rwork | 0.195 |
| R-free | 0.24861 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8bjv |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.406 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (v7.0.28) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.470 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmeas | 0.077 | 0.445 |
| Rpim | 0.018 | 0.099 |
| Total number of observations | 360632 | 55510 |
| Number of reflections | 19078 | 2767 |
| <I/σ(I)> | 23 | 7 |
| Completeness [%] | 100.0 | |
| Redundancy | 18.9 | 20.1 |
| CC(1/2) | 0.999 | 0.985 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 30.00 % w/v Polyethylene glycol 4,000, 100 mM tri-Sodium citrate; pH 5.6, 100 mM Ammonium sulfate3/D |
