8BPM

Crystal structure of Trichoplax Dlg PDZ3 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-11-29
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 1
Unit cell lengths	32.375, 35.434, 72.990
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.420 - 2.800
R-factor	0.2098
Rwork	0.208
R-free	0.24570
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3i4w
RMSD bond length	0.005
RMSD bond angle	0.733
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.420	2.950
High resolution limit [Å]	2.800	2.800
Number of reflections	7852	1188
<I/σ(I)>	4.1
Completeness [%]	98.2
Redundancy	3.6
CC(1/2)	0.975	0.821

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	30% w/v PEG 1500