8BOJ

Crystal structure of Trichoplax Scribble PDZ1 domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-12
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.95372
Spacegroup name	P 21 21 21
Unit cell lengths	24.067, 49.016, 68.873
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	39.930 - 1.500
R-factor	0.1829
Rwork	0.181
R-free	0.20940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5vwc
RMSD bond length	0.004
RMSD bond angle	0.710
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.020	1.530
High resolution limit [Å]	1.500	1.500
Rmerge	0.105	0.906
Number of reflections	13564	657
<I/σ(I)>	16	3.8
Completeness [%]	99.1
Redundancy	13
CC(1/2)	0.999	0.906

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	1.4 M sodium citrate, pH 5.7