8BMU

Engineered Fructosyl Peptide Oxidase - X04 mutant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X06DA
Synchrotron site	SLS
Beamline	X06DA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-08
Detector	PSI PILATUS 6M
Wavelength(s)	1.0
Spacegroup name	P 42 21 2
Unit cell lengths	90.238, 90.238, 131.437
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.820 - 1.600
R-factor	0.1669
Rwork	0.165
R-free	0.19411
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6y4j
RMSD bond length	0.010
RMSD bond angle	1.685
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	74.393	1.673
High resolution limit [Å]	1.600	1.600
Number of reflections	62950	2416
<I/σ(I)>	20.5	1.1
Completeness [%]	94.7
Redundancy	16.1
CC(1/2)	0.999	0.458

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	293	PEG 20000 16-26%, MES 0.1M pH 6.5