8BLV

The PDZ domains of human SDCBP with a bound SDC4 C-terminal peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I03
Synchrotron site	Diamond
Beamline	I03
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-21
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.9763
Spacegroup name	P 21 21 21
Unit cell lengths	56.710, 67.462, 106.740
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	57.027 - 1.500
Rwork	0.182
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1n99
RMSD bond length	0.013
RMSD bond angle	1.729
Data reduction software	DIALS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	106.740	1.530
High resolution limit [Å]	1.500	1.500
Rpim	0.033	2.217
Number of reflections	66371	3237
<I/σ(I)>	15.2	0.6
Completeness [%]	100.0
Redundancy	79.2
CC(1/2)	0.999	0.658

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	293	200 mM sodium sulphate, 100 mM bis-tris-propane, 10% ethylene glycol, 20% PEG 3350