8BLT
Structure of Lactobacillus salivarius (Ls) bile salt hydrolase(BSH) in complex with taurocholate (TCA)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU FR-E+ SUPERBRIGHT |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-05-01 |
| Detector | RIGAKU SATURN 944 |
| Wavelength(s) | 1.54 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 84.759, 94.605, 168.215 |
| Unit cell angles | 90.00, 90.26, 90.00 |
Refinement procedure
| Resolution | 25.750 - 2.100 |
| R-factor | 0.18158 |
| Rwork | 0.180 |
| R-free | 0.21264 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5hke |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.629 |
| Data reduction software | iMOSFLM |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.140 |
| High resolution limit [Å] | 2.100 | 2.100 |
| Rmeas | 0.105 | 0.340 |
| Number of reflections | 150183 | 150183 |
| <I/σ(I)> | 10.7 | |
| Completeness [%] | 96.7 | |
| Redundancy | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 4.8 | 293 | 20% Polyethylene glycol 3350, 0.2M potassium dihydrogen phosphate pH 4.8 |
