8BL9
Crystal Structure of Sam0.7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-09-25 |
| Detector | DECTRIS EIGER2 X 16M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 40.554, 40.554, 253.117 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 35.120 - 1.800 |
| Rwork | 0.257 |
| R-free | 0.29610 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | computational model |
| RMSD bond length | 0.023 |
| RMSD bond angle | 1.959 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0266) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.120 | 1.910 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.103 | 1.623 |
| Number of reflections | 12505 | 1934 |
| <I/σ(I)> | 19 | 1.65 |
| Completeness [%] | 100.0 | 99.9 |
| Redundancy | 32.77 | 34.94 |
| CC(1/2) | 0.999 | 0.965 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 294 | 20% w/v PEG3350, 200 mM KSCN |
