8BKE
Crystal structure of the Klebsiella phage KP34p57 capsular depolymerase
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM30A |
| Synchrotron site | ESRF |
| Beamline | BM30A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-02-14 |
| Detector | DECTRIS PILATUS3 2M |
| Wavelength(s) | 0.987 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 38.340, 140.985, 81.925 |
| Unit cell angles | 90.00, 102.63, 90.00 |
Refinement procedure
| Resolution | 15.000 - 2.000 |
| Rwork | 0.204 |
| R-free | 0.24730 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.529 |
| Data reduction software | HKL-2000 (HKL2000_712-Linux) |
| Data scaling software | HKL-2000 (HKL2000_712-Linux) |
| Phasing software | SOLVE (2.13) |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.250 |
| High resolution limit [Å] | 2.000 | 2.200 |
| Rmerge | 0.110 | 0.410 |
| Number of reflections | 203918 | 2652 |
| <I/σ(I)> | 14.2 | |
| Completeness [%] | 87.1 | 91.3 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.4 | 293 | 0.04 M cytric acid, 0.06 M bis-tris propane, 20% PEG 3350 |
