8BJS

Apo KIF20A[55-510] crystal structure

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-01-26
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.979
Spacegroup name	C 1 2 1
Unit cell lengths	132.438, 48.490, 85.836
Unit cell angles	90.00, 124.41, 90.00

Refinement procedure

Resolution	54.630 - 2.730
R-factor	0.21
Rwork	0.206
R-free	0.24300
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Se-SAD Structure
RMSD bond length	0.008
RMSD bond angle	1.010
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	64.240	2.830
High resolution limit [Å]	2.730	2.730
Rmerge	0.133	0.678
Rmeas	0.144	0.731
Number of reflections	12169	1209
<I/σ(I)>	9.5	2.35
Completeness [%]	99.8
Redundancy	6.9
CC(1/2)	0.995	0.887

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	1.8 M NaCl, 0.5 M (NH4)2SO4, 0.1 M Bis-Tris, pH 5.5