8BH8
Structure of Est1 from Candida Tropicalis in complex with TLC1 telomerase RNA fragment 444-456
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U1 |
| Synchrotron site | SSRF |
| Beamline | BL17U1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2017-04-30 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.978880 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 36.300, 79.069, 108.431 |
| Unit cell angles | 90.00, 96.25, 90.00 |
Refinement procedure
| Resolution | 36.080 - 2.880 |
| R-factor | 0.2239 |
| Rwork | 0.221 |
| R-free | 0.27940 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.743 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | SHARP |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 44.533 | 2.926 |
| High resolution limit [Å] | 2.876 | 2.876 |
| Rmerge | 0.068 | 0.520 |
| Rmeas | 0.084 | 0.637 |
| Rpim | 0.049 | 0.364 |
| Number of reflections | 13852 | 705 |
| <I/σ(I)> | 10.9 | 2 |
| Completeness [%] | 98.9 | 99.9 |
| Redundancy | 2.8 | 3 |
| CC(1/2) | 0.998 | 0.795 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 298 | PEG 4K 20% PEG 200 5% Tris-HCl 0.1 M PH 7.5 |
