8BGT
O-Methyltransferase Plu4890 in complex with SAM
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06SA |
| Synchrotron site | SLS |
| Beamline | X06SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2017-11-04 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 63.260, 76.780, 169.150 |
| Unit cell angles | 90.00, 96.67, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.150 |
| R-factor | 0.2252 |
| Rwork | 0.223 |
| R-free | 0.26550 |
| Structure solution method | SAD |
| RMSD bond length | 0.002 |
| RMSD bond angle | 1.156 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | CRANK2 |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.000 | 2.250 |
| High resolution limit [Å] | 2.150 | 2.150 |
| Rmerge | 0.044 | 0.517 |
| Number of reflections | 42753 | 5475 |
| <I/σ(I)> | 10.9 | 2.1 |
| Completeness [%] | 97.4 | 98.2 |
| Redundancy | 2.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | 0.2 M NaF, 0.1 M bis-tris propane pH 7.5, 20% PEG3350 |
