8BGR

FAD-independent Methylene-Tetrahydrofolate Reductase from Mycobacterium hassiacum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SLS BEAMLINE X10SA
Synchrotron site	SLS
Beamline	X10SA
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-04
Detector	DECTRIS EIGER2 X 16M
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	45.790, 45.790, 257.410
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.310 - 1.800
R-factor	0.2331
Rwork	0.232
R-free	0.24940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold Model
RMSD bond length	0.003
RMSD bond angle	0.597
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.900
High resolution limit [Å]	1.800	1.800
Rmeas	0.104	1.300
Number of reflections	26845	3422
<I/σ(I)>	9	2
Completeness [%]	94.9
Redundancy	4.3
CC(1/2)	0.996	0.851

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		281	15% PEG 6000