8BFT

The E. coli TrpD2 protein YbiB in complex with a C-terminal peptide from ObgE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	133.469, 63.895, 47.416
Unit cell angles	90.00, 97.35, 90.00

Refinement procedure

Resolution	47.030 - 1.190
R-factor	0.1382
Rwork	0.137
R-free	0.16680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8bfr
RMSD bond length	0.009
RMSD bond angle	1.051
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	1.331
High resolution limit [Å]	1.190	1.192
Number of reflections	87511	4374
<I/σ(I)>	9.4
Completeness [%]	92.8
Redundancy	6.8
CC(1/2)	0.997	0.696

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M Citrate pH 5, 20% w/v PEG6000