8BFR

The E. coli TrpD2 protein YbiB at 1.3 A resolution

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SOLEIL BEAMLINE PROXIMA 1
Synchrotron site	SOLEIL
Beamline	PROXIMA 1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-07
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97856
Spacegroup name	C 1 2 1
Unit cell lengths	134.154, 64.004, 47.496
Unit cell angles	90.00, 97.22, 90.00

Refinement procedure

Resolution	66.550 - 1.300
R-factor	0.1366
Rwork	0.135
R-free	0.16670
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4muo
RMSD bond length	0.009
RMSD bond angle	1.087
Data reduction software	autoPROC (1.0.5)
Data scaling software	autoPROC (1.0.5)
Phasing software	PHENIX (1.20.1_4487)
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	66.550	1.397
High resolution limit [Å]	1.300	1.300
Number of reflections	80496	4026
<I/σ(I)>	10.8
Completeness [%]	93.6
Redundancy	6.8
CC(1/2)	0.998	0.624

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293.15	0.1 M MES pH 6.2, 15 % w/v PEG 3350