8BEY
Structure of the Lysinibacillus sphaericus Tpp49Aa1 pesticidal protein at pH 7
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | EUROPEAN XFEL BEAMLINE SPB/SFX |
| Synchrotron site | European XFEL |
| Beamline | SPB/SFX |
| Temperature [K] | 298 |
| Detector technology | PIXEL |
| Collection date | 2022-04-22 |
| Detector | AGIPD |
| Wavelength(s) | 1.33 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 79.650, 83.110, 156.910 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.980 - 1.620 |
| R-factor | 0.1771 |
| Rwork | 0.176 |
| R-free | 0.19470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wa1 5foy 5g37 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.439 |
| Data reduction software | CrystFEL |
| Data scaling software | CrystFEL (Ambigator) |
| Phasing software | PHASER (2.8.3) |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.980 | 1.640 |
| High resolution limit [Å] | 1.620 | 1.620 |
| Number of reflections | 132750 | 8756 |
| <I/σ(I)> | 10.61 | |
| Completeness [%] | 100.0 | |
| Redundancy | 1028.3 | |
| CC(1/2) | 0.997 | 0.133 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 7 | 298 | Natural |
