8BEN
LRR domain Structure of the LRRC8C protein
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SLS BEAMLINE X10SA |
Synchrotron site | SLS |
Beamline | X10SA |
Temperature [K] | 80 |
Detector technology | PIXEL |
Collection date | 2017-02-23 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 73.376, 104.726, 111.829 |
Unit cell angles | 90.00, 94.71, 90.00 |
Refinement procedure
Resolution | 49.190 - 3.100 |
R-factor | 0.2438 |
Rwork | 0.241 |
R-free | 0.28960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6fnw |
RMSD bond length | 0.007 |
RMSD bond angle | 0.841 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHENIX |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.190 | 3.200 |
High resolution limit [Å] | 3.100 | 3.100 |
Rmerge | 0.350 | |
Number of reflections | 30438 | 2773 |
<I/σ(I)> | 5.53 | |
Completeness [%] | 98.3 | |
Redundancy | 7.2 | |
CC(1/2) | 0.760 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 277.15 | 0.2 M magnesium chloride, 20% PEG3350 |