8BDY
Crystal structure of TRIM33 alpha PHD-Bromo domain in complex with 9
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I04 |
| Synchrotron site | Diamond |
| Beamline | I04 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-05-13 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 79.965, 79.965, 135.497 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 69.250 - 3.050 |
| R-factor | 0.23379 |
| Rwork | 0.229 |
| R-free | 0.33422 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3u5m |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.771 |
| Data reduction software | XDS |
| Data scaling software | SCALA |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 135.500 | 3.210 |
| High resolution limit [Å] | 3.050 | 3.050 |
| Rmerge | 0.182 | 1.079 |
| Rmeas | 0.189 | 1.118 |
| Rpim | 0.051 | 0.290 |
| Number of reflections | 5355 | 761 |
| <I/σ(I)> | 10 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 13.6 | 14.7 |
| CC(1/2) | 0.995 | 0.827 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 281 | 0.2 M calcium chloride and 20 % wt/vol PEG-3350 |
