8BDB
Ribulose-1,5-bisphosphate carboxylase/oxygenase from Griffithsia monilis
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I02 |
| Synchrotron site | Diamond |
| Beamline | I02 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-15 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9795 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 210.328, 131.925, 196.319 |
| Unit cell angles | 90.00, 94.75, 90.00 |
Refinement procedure
| Resolution | 49.600 - 1.700 |
| R-factor | 0.1588 |
| Rwork | 0.158 |
| R-free | 0.17800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1bwv |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.276 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 49.600 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Rmerge | 0.101 | 0.891 |
| Rmeas | 0.139 | 1.238 |
| Rpim | 0.095 | 0.857 |
| Number of reflections | 578867 | 25785 |
| <I/σ(I)> | 7.6 | 0.9 |
| Completeness [%] | 99.2 | 89.7 |
| Redundancy | 3.3 | 2.4 |
| CC(1/2) | 0.995 | 0.360 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | HEPES, lithium sulphate, PEG 3350 |
