8BDB

Ribulose-1,5-bisphosphate carboxylase/oxygenase from Griffithsia monilis

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I02
Synchrotron site	Diamond
Beamline	I02
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-10-15
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.9795
Spacegroup name	C 1 2 1
Unit cell lengths	210.328, 131.925, 196.319
Unit cell angles	90.00, 94.75, 90.00

Refinement procedure

Resolution	49.600 - 1.700
R-factor	0.1588
Rwork	0.158
R-free	0.17800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1bwv
RMSD bond length	0.007
RMSD bond angle	1.276
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0107)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.600	1.730
High resolution limit [Å]	1.700	1.700
Rmerge	0.101	0.891
Rmeas	0.139	1.238
Rpim	0.095	0.857
Number of reflections	578867	25785
<I/σ(I)>	7.6	0.9
Completeness [%]	99.2	89.7
Redundancy	3.3	2.4
CC(1/2)	0.995	0.360

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	HEPES, lithium sulphate, PEG 3350