8BD0

Human Gamma-D crystallin R36S mutant with DTT-Cystein Protein modification

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PETRA III, EMBL c/o DESY BEAMLINE P14 (MX2)
Synchrotron site	PETRA III, EMBL c/o DESY
Beamline	P14 (MX2)
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-12-01
Detector	DECTRIS EIGER X 1M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.900, 83.700, 101.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	101.000 - 2.000
R-factor	0.20727
Rwork	0.204
R-free	0.24971
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2g98
RMSD bond length	0.008
RMSD bond angle	1.492
Data reduction software	CrystFEL
Data scaling software	CrystFEL
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	101.130	2.070
High resolution limit [Å]	2.000	2.000
Number of reflections	31629	3090
<I/σ(I)>	2.71	0.6
Completeness [%]	100.0	100
Redundancy	50.3	34.7
CC(1/2)	0.996	0.140

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	BATCH MODE	7.8	298	50 mM phosphate buffer pH 7.8, 20 mM DTT