8BCX
Crystal structure of NrfA-1 from Geobacter metallireducens
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-25 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.734 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 91.085, 91.965, 123.939 |
| Unit cell angles | 90.00, 99.06, 90.00 |
Refinement procedure
| Resolution | 122.390 - 1.941 |
| R-factor | 0.1945 |
| Rwork | 0.193 |
| R-free | 0.22720 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.870 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | CRANK2 |
| Refinement software | BUSTER (2.10.4 (8-JUN-2022)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 122.390 | 2.170 |
| High resolution limit [Å] | 1.940 | 1.940 |
| Number of reflections | 92671 | 4634 |
| <I/σ(I)> | 13.5 | |
| Completeness [%] | 62.1 | |
| Redundancy | 53.5 | |
| CC(1/2) | 0.999 | 0.855 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 0.1 M BIS-TRIS pH 5.5, 0.1 M ammonium sulfate, 22.5 % PEG 3350 |
