8BC5

Recombinant Tipula oleracea Nudivirus Polyhedrin - Selenomethionine

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE VMXm
Synchrotron site	Diamond
Beamline	VMXm
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-13
Detector	DECTRIS EIGER2 S 9M
Wavelength(s)	0.97873
Spacegroup name	P 32 2 1
Unit cell lengths	53.481, 53.481, 105.248
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.390 - 1.910
R-factor	0.1715
Rwork	0.169
R-free	0.21720
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.769
Data reduction software	xia2.multiplex
Data scaling software	xia2
Phasing software	CRANK
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	42.390	1.978
High resolution limit [Å]	1.910	1.910
Number of reflections	13277	1129
<I/σ(I)>	24.64
Completeness [%]	93.9	79.74
Redundancy	51.7
CC(1/2)	0.950	0.275

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	IN CELL		301	In cell crystallisation