Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I24 |
| Synchrotron site | Diamond |
| Beamline | I24 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-11-22 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.999 |
| Spacegroup name | P 65 2 2 |
| Unit cell lengths | 109.470, 109.470, 84.590 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 47.402 - 2.200 |
| Rwork | 0.215 |
| R-free | 0.25960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2vb2 |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.558 |
| Data reduction software | DIALS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.402 | 2.279 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 15531 | 1502 |
| <I/σ(I)> | 15.1 | |
| Completeness [%] | 98.9 | |
| Redundancy | 36.9 | |
| CC(1/2) | 1.000 | 0.600 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.01 M Zinc sulphate heptahydrate, 0.1 M MES pH 6.5, 25% v/v PEG 550 MME |
