8BBL
SGL a GH20 family sulfoglycosidase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | DIAMOND BEAMLINE I03 |
| Synchrotron site | Diamond |
| Beamline | I03 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-01-17 |
| Detector | DECTRIS EIGER2 XE 16M |
| Wavelength(s) | 0.9762 |
| Spacegroup name | P 1 |
| Unit cell lengths | 132.950, 133.420, 225.370 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 94.176 - 2.711 |
| Rwork | 0.283 |
| R-free | 0.31870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | alphafold |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.761 |
| Data reduction software | xia2 |
| Data scaling software | xia2 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0352) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 94.180 | 2.770 |
| High resolution limit [Å] | 2.710 | 2.710 |
| Number of reflections | 406028 | 20183 |
| <I/σ(I)> | 5.1 | 1.6 |
| Completeness [%] | 97.6 | 97.8 |
| Redundancy | 1.9 | |
| CC(1/2) | 0.981 | 0.343 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | 23.7 mg/mL protein in the buffer of Tris-HCl 25 mM, pH 8.0, and NaCl 200 mM was mixed 1:1 with the precipitant containing 0.1 M Bis-tris, pH 6.5, and 18-21.5% w/v PEG5000 MME. |
