8BAZ
The surface-exposed lipo-protein of BtuG2 in complex with cyanocobalamin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 49.177, 100.593, 79.579 |
| Unit cell angles | 90.00, 97.60, 90.00 |
Refinement procedure
| Resolution | 44.199 - 2.000 |
| Rwork | 0.181 |
| R-free | 0.24320 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6FFV |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.806 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0415) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.890 | 8.000 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Number of reflections | 51840 | 40883 |
| <I/σ(I)> | 14 | |
| Completeness [%] | 99.0 | |
| Redundancy | 8 | |
| CC(1/2) | 0.960 | 0.940 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 298 | 50 mM TRIS pH, 100 mM NaCl, 20 mM ZnCl, 20 mM Cyanocobalamin, PEG2000 20% |
