8BAI
The surface-exposed lipo-protein of BtuG2 in complex with cyanocobalamin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID23-1 |
| Synchrotron site | ESRF |
| Beamline | ID23-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-11-01 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 49.240, 79.936, 101.047 |
| Unit cell angles | 89.97, 89.89, 82.28 |
Refinement procedure
| Resolution | 42.630 - 1.690 |
| R-factor | 0.15723 |
| Rwork | 0.157 |
| R-free | 0.18007 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1nqg |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.006 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0405) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 101.100 | 8.600 |
| High resolution limit [Å] | 1.690 | 1.690 |
| Number of reflections | 169723 | 169700 |
| <I/σ(I)> | 18 | |
| Completeness [%] | 99.0 | |
| Redundancy | 6 | |
| CC(1/2) | 0.980 | 0.980 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | TRIS pH 8.0, PEG4000, NaCl, MgCl |
