8B8A

Multimerization domain of borna disease virus 1 phosphoprotein

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID29
Synchrotron site	ESRF
Beamline	ID29
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-24
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97717
Spacegroup name	P 4 21 2
Unit cell lengths	35.271, 35.271, 166.512
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.750
R-factor	0.2578
Rwork	0.255
R-free	0.30700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4bhv
RMSD bond length	0.009
RMSD bond angle	0.970
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	BUSTER (2.10.3 (3-OCT-2019))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	55.000	2.820
High resolution limit [Å]	2.750	2.750
Rmerge	0.258	3.260
Rpim	0.108	1.357
Number of reflections	3173	214
<I/σ(I)>	5
Completeness [%]	99.9	99.7
Redundancy	11.1	11.8
CC(1/2)	0.998	0.601

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.4	293	100 mM HEPES pH 7.5, 14-18 % polyethylene glycol 8K, 200 mM CaCl2