8B8A
Multimerization domain of borna disease virus 1 phosphoprotein
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-08-24 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97717 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 35.271, 35.271, 166.512 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.750 |
| R-factor | 0.2578 |
| Rwork | 0.255 |
| R-free | 0.30700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4bhv |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.970 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | BUSTER (2.10.3 (3-OCT-2019)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 55.000 | 2.820 |
| High resolution limit [Å] | 2.750 | 2.750 |
| Rmerge | 0.258 | 3.260 |
| Rpim | 0.108 | 1.357 |
| Number of reflections | 3173 | 214 |
| <I/σ(I)> | 5 | |
| Completeness [%] | 99.9 | 99.7 |
| Redundancy | 11.1 | 11.8 |
| CC(1/2) | 0.998 | 0.601 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | 100 mM HEPES pH 7.5, 14-18 % polyethylene glycol 8K, 200 mM CaCl2 |
