8B81
The structure of Gan1D W433A in complex with cellobiose-6-phosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-15 |
| Detector | DECTRIS PILATUS 6M-F |
| Wavelength(s) | 0.987 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 107.900, 68.380, 149.800 |
| Unit cell angles | 90.00, 99.36, 90.00 |
Refinement procedure
| Resolution | 49.269 - 1.585 |
| R-factor | 0.1697 |
| Rwork | 0.169 |
| R-free | 0.20130 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 8b80 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.680 |
| High resolution limit [Å] | 1.585 | 1.585 |
| Rmerge | 0.104 | 0.588 |
| Number of reflections | 141155 | 20970 |
| <I/σ(I)> | 8.7 | 1.82 |
| Completeness [%] | 96.8 | 89.2 |
| Redundancy | 5.1 | 5.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 19% polyethylene glycol 8K, 3% MPD, 0.1 M imidazole buffer pH 6.5 |
