Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ELETTRA BEAMLINE 11.2C |
| Synchrotron site | ELETTRA |
| Beamline | 11.2C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-03-04 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 1.00 |
| Spacegroup name | F 4 3 2 |
| Unit cell lengths | 181.990, 181.990, 181.990 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 64.340 - 1.240 |
| Rwork | 0.158 |
| R-free | 0.17980 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5erk |
| RMSD bond length | 0.020 |
| RMSD bond angle | 2.243 |
| Data reduction software | autoPROC |
| Data scaling software | autoPROC |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 64.340 | 1.260 |
| High resolution limit [Å] | 1.240 | 1.240 |
| Rmerge | 0.122 | 1.800 |
| Rpim | 0.014 | 0.364 |
| Number of reflections | 88885 | 3593 |
| <I/σ(I)> | 34.9 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 20 | 15 |
| CC(1/2) | 1.000 | 0.772 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.2 | 293 | 0.8 M ammonium sulphate 0.1 M TrisHCl pH 7.4 60 mM cadmium sulphate |
