8B7F
Nuclease from C. glutamicum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SOLEIL BEAMLINE PROXIMA 1 |
| Synchrotron site | SOLEIL |
| Beamline | PROXIMA 1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2022-06-17 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 0.9786 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 78.086, 126.776, 150.111 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.210 - 4.600 |
| R-factor | 0.2717 |
| Rwork | 0.270 |
| R-free | 0.31140 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | AlphaFold2 |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.887 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1-4487-000) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.500 | 4.770 |
| High resolution limit [Å] | 4.600 | 4.600 |
| Rpim | 0.054 | 0.444 |
| Number of reflections | 8738 | 597 |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 99.5 | |
| Redundancy | 13.1 | |
| CC(1/2) | 0.998 | 0.816 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 291 | 10% (w/v) PEG 6K, 30% (v/v) ethanol and 10 mM sodium acetate |
