8B76
The crystal structure of M644G variant of DNA Pol Epsilon containing dTTP in the polymerase active site
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID29 |
| Synchrotron site | ESRF |
| Beamline | ID29 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-09-03 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97625 |
| Spacegroup name | P 1 2 1 |
| Unit cell lengths | 154.160, 69.880, 159.050 |
| Unit cell angles | 90.00, 112.63, 90.00 |
Refinement procedure
| Resolution | 86.830 - 2.600 |
| R-factor | 0.2217 |
| Rwork | 0.220 |
| R-free | 0.24680 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4m8o |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 86.830 | 86.830 | 2.640 |
| High resolution limit [Å] | 2.600 | 14.240 | 2.600 |
| Rmerge | 0.092 | 0.037 | 0.684 |
| Rmeas | 0.111 | 0.046 | 0.825 |
| Rpim | 0.062 | 0.027 | 0.456 |
| Total number of observations | 291431 | 1823 | 14771 |
| Number of reflections | 96267 | 604 | 4743 |
| <I/σ(I)> | 7.4 | 16.8 | 1.5 |
| Completeness [%] | 99.5 | 91.7 | 99.8 |
| Redundancy | 3 | 3 | 3.1 |
| CC(1/2) | 0.995 | 0.982 | 0.416 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 292 | 10mM Tris, 15% PEG8K,10mM CaCl2 |
