8B74

Tyrosinase from halophilic bacterium Hahella sp. CCB MM4

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE ID30B
Synchrotron site	ESRF
Beamline	ID30B
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-04-29
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97625
Spacegroup name	P 2 2 21
Unit cell lengths	51.140, 55.572, 156.298
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	78.271 - 1.965
Rwork	0.175
R-free	0.22620
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold
RMSD bond length	0.015
RMSD bond angle	2.111
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0352)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	78.271	1.999
High resolution limit [Å]	1.965	1.965
Rmerge	0.081	0.565
Number of reflections	32382	1605
<I/σ(I)>	9.7
Completeness [%]	99.2	100
Redundancy	3.7	3.7
CC(1/2)	0.997	0.781

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	300	0.2 M LiSO4, 0.1 Bis-Tris pH 5.5, 20 % (w/v) PEG 4000