8B5X

Crystal structure of the SUN1-KASH6 9:6 complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	DIAMOND BEAMLINE I24
Synchrotron site	Diamond
Beamline	I24
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-07-16
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.6702
Spacegroup name	P 63
Unit cell lengths	133.921, 133.921, 106.581
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	32.170 - 1.980
R-factor	0.1753
Rwork	0.173
R-free	0.21070
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8b46
RMSD bond length	0.008
RMSD bond angle	1.095
Data reduction software	autoPROC
Data scaling software	STARANISO
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	115.980	2.221
High resolution limit [Å]	1.980	1.984
Rmerge	0.215	2.107
Rpim	0.048	0.458
Number of reflections	43359	2170
<I/σ(I)>	12.5	1.8
Completeness [%]	57.8	10.1
Redundancy	21.2
CC(1/2)	0.998	0.663

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.15 M sodium acetate; 0.1 M Sodium citrate tribasic dihydrate pH 5.5 (+25% ethylene glycol cryoprotectant)